Immune islet killing mechanisms associated with insulin-dependent diabetes: Three rabbit antibody-mediated islet cell cytotoxicity models
نویسندگان
چکیده
منابع مشابه
Complement activation pathways associated with islet cell surface antibody (ICSA) derived from child patients with insulin-dependent diabetes mellitus (IDDM).
We studied the pathways of complement activation associated with the islet cell surface antibody (ICSA) obtained from sera of 7 patients (age less than 15 years) with insulin dependent diabetes mellitus (IDDM). The target cells were 51CR labelled rat islet cells and the complement source was human AB serum. Complement-dependent antibody mediated cytotoxicity (CAMC activity) was obtained using t...
متن کاملMacrophage-mediated islet cell cytotoxicity in BB rats.
Islet cell killing mediated by natural killer cells and T-lymphocytes in diabetes-prone (DP) and diabetic BB rats has been described, but other killing mechanisms may also be involved. Histopathologic studies suggest that macrophages are the first immune cells to infiltrate islets. To determine if macrophages are the first cells mediating islet damage, macrophage-mediated cytotoxicity was evalu...
متن کاملProtective role of human insulin against the cytotoxicity associated with human mutant S20G islet amyloid polypeptide
AIMS/INTRODUCTION Islet amyloid polypeptide (IAPP) is a main component of islet amyloid in type 2 diabetes and cosecreted from β-cell with insulin. Clinical evidence from the patients with S20G mutation of the IAPP gene, as well as experimental evidence that insulin could inhibit amyloid formation of IAPP, suggests that a gradual reduction of insulin could be related to the cytotoxicity associa...
متن کاملIslet cell cytoplasmic autoantibody reactivity to glutamate decarboxylase in insulin-dependent diabetes.
Individuals with or at risk for insulin-dependent diabetes (IDD) frequently have autoantibodies against an islet cell cytoplasmic (ICA) antigen thought to be a sialoglycolipid. However, we now report that preabsorption of ICA-positive sera with recombinant glutamate decarboxylase (human GAD 65 and/or GAD 67) reduced or blocked the ICA reactivity of 5/18 (27%) new-onset IDD patients and 7/18 (39...
متن کاملIslet Biology/Insulin Secretion
duces proinflammatory lipids that exacerbate β cell dysfunction and death. Inhibition of 12/15-LOX thereby provides a potential therapeutic approach to prevent glycemic deterioration in T1D, but to date no specific inhibitors have been tested T1D. Here we assessed the efficacy and mechanism of a novel and specific 12/15-LOX inhibitor, ML351. Using cultured β cells in vitro, ML351 exhibited no a...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Diabetologia
سال: 1983
ISSN: 0012-186X,1432-0428
DOI: 10.1007/bf00253200